Biochem/physiol Actions

Primary TargetRatiometric calcium indicator

Reversible: no

Product does not compete with ATP.

Cell permeable: yes

General description

PLURONIC^® F-127, PROTEIN GRADE™ Detergent (Cat. No. 540025, available separately) is a mild, low-toxicity detergent that can aid in the solubilization of FURA 2/AM.

Cell-permeable ester form of FURA 2. Note: 1 set = 10 x 100 µg.

Cell permeant ester form of FURA 2 (Cat. No. 344900). The active dye is rapidly released in the cell by cytoplasmic esterases. Fluorescent calcium chelator (K_d = 224 nM) that offers 30 times greater fluorescence intensity than QUIN 2 (Cat. No. 551826). Can be used as a probe in many cellular systems, including cardiac myocytes, vascular smooth muscle cells, and medullary collecting tubules. Spectral properties in the presence of low calcium concentrations (excitation max.: 362 nm, emission max.: 512 nm) differ from those during high calcium conditions (excitation max.: 335 nm, emission max.: 505 nm). Note: 1 set = 10 x 100 µg.

Legal Information

Pluronic is a registered trademark of BASF

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Davis, M.J., et al. 1992. Am. J. Physiol. 263, H1292.Rao, G.H., et al. 1992. Biochem. Med. Metab. Biol.47, 226.Schwartz, Y., et al. 1992. Endocrinology131, 772.Ward, C.A., and Moffat, M.P. 1992. J. Mol. Cell. Cardiol.24, 937.Janconi, M.E., et al. 1990. J. Cell Biol.110, 1555.Tsien, R.Y. 1980. Biochemistry19, 2396.

Packaging

1 set in Glass bottle

1 mg in Plastic ampoule

Packaged under inert gas

Preparation Note

Further dilute with aqueous buffers just prior to use.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C) under an inert gas. Stock solutions are stable for up to 1 month at -20°C.

Warning

Toxicity: Standard Handling (A)